Course Coordinator: Assistant Professor ZHANG Li-Feng (email: ZHANGLF@ntu.edu.sg)
Academic Unit: 3 AU
Availability: Semester 1
Pre-requisite: BS1006, BS2004
Course Type: BS-Major PE, BMS-Major-PE
Language of instruction: English
Teaching hours: Lectures: 24 hours; Tutorials: 11 hours
Learning Objective: BS3005 lecture series provide students with theoretical knowledge of key experimental techniques involved in modern molecular genetic studies. It also introduces students to the important genetic model systems, such as mouse, yeast, fruit fly and Arabidopsis.
Content: Following the main theme “how to generate a genetically engineered mouse”, the lecture series cover most of the key experimental techniques involved in modern molecular genetic research. How to amplify, clone and modify DNA fragments in test tubes? How to introduce engineered DNA fragment into the genome of a mouse cell? How to generate a mouse from an embryonic stem (ES) cell with engineered genome? From there on, the topics are switched to ES cells and epigenetics, two important topics in current research. At the end of the lecture series, the students are introduced with the concept of genetic screen and important genetic model systems, such as yeast, fruit fly, Arabidopsis and mouse.
Learning Outcome: After completion of this course, students are expected to be able to:
- Design simple experiments to manipulate short DNA fragments in test tubes
- Understand a given experimental design of manipulating large DNA fragments
- Understand important concepts in current embryonic stem cell studies and epigenetic studies
- List a few famous studies done with genetic model systems
- Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Molecular Biology of the Cell, 5th Edition, Garland Science, c2008 (QH581.2.M718), ISBN 978-0-8153-4105-5 Paperback Book
*Prerequisites for Incoming Exchange Students: Equivalent of Year 2 or Higher Biology or Biotechnology major