Curriculum & Course Descriptions

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BS7006 Practical Course in Protein Crystallography

Course Coordinator: Associate Professor Julien LESCAR (email: JULIEN@ntu.edu.sg)

Availability: Semester 1

The aim of this course is to provide a hands-on approach to practical modern biological macromolecular crystallography. Lectures in the morning are followed by practical classes at the bench or in the computer lab. The intended audience is composed of graduates, who want to use crystallographic methods during the course of their PhD projects and/or PhD students in the field of crystallography.

Topics:

  • What use is Structural Biology?
  • How to grow crystals
  • How to analyse, optimize, and soak crystals
  • How to harvest and freeze the crystal trials
  • How to collect data
  • How to process data
  • How to solve structures by molecular replacement
  • How to phase SAD/MAD – sulfur, selenium, halides, metals
  • How to build a model
  • How to evaluate the model
  • How to interpret the model and connection with other results (SAXS, EM, biochemistry)