- B.Sc., Biotechnology, Zhejiang University, China(2002)
- M.phil., Biology, The Chinese University of Hong Kong, Hong Kong (2006)
- Ph.D., Molecular Biotechnology, The Chinese University of Hong Kong, Hong Kong (2009)
- Assistant Professor (2015-present), School of Biological Science (SBS) & School of Chemical and Biomedical Engineering (SCBE), Nanyang Technological University, Singapore
- Human Frontier Science Program Postdoc Long-term Fellow (2010-2013), University of California, Berkeley, CA, US
- Postdoctoral Fellow (2010-2014), University of California, Berkeley, CA, US
Our lab is interested in how eukaryotic cells dynamically reorganize their actin cytoskeletons to power endocytosis, polarized delivery of vesicles for cell growth, cell movement, and cell division. Our research is focus on understanding of how different actin interacting proteins (AIPs) are regulated to assemble different types of actin arrays, nucleated by Arp2/3 complex and formins, in the same cytoplasm during these processes, and at different stages of the cell cycle.
We are using multi-disciplinary approach including reconstitution of actin assembly in cell extracts, live-cell imaging, genetics, biochemistry, super-resolution microscopy, and mass spectrometry to address these questions from the molecular to cellular level. Our working systems include budding yeast, plants, and cultured mammalian cell lines.
One of our major goals is to determine the function and molecular fundamentals of several key AIPs that regulate the dynamics of different actin arrays at specific cell cycle stage or a particular physiological conditions, e.g. stress response. And we also aim to reconstitute such actin assembly by our cell-extract based system at these particular conditions.
Reconstitution of the actin cable assembly using formin-
coated microspheres in cell-free extract