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Geifman Shochat, Susana

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Geifman Shochat, Susana

Geifman Shochat, Susana
Associate Professor

Office: 04s-45
Telephone: 6513 8023
Email: SGeifman@ntu.edu.sg

 

Education

  • BSc. (Chemistry) The Hebrew University, Jerusalem, Israel (1973)
  • MSc. (Biochemistry)The Hebrew University, Jerusalem, Israel (1975)
  • PhD. (Biophysics) Leiden University, The Netherlands (1996)

Professional Experience

  • 1975-1989 Technical laboratory officer (MSc). Hebrew University of Jerusalem.
  • 1990-1994 Doctoral position, Department of Biophysics, Leiden University, NL.
  • 1995-1998 Technical laboratory officer (PhD) The Hebrew University of Jerusalem
  • 1998-2003 Director for Biacore Core Facility. The Hebrew University of Jerusalem
  • 2004 Research scientist, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC-Chambon Institute) Illkirch, France.
  • 2005-2007 Research scientist, Ecole Superieur de Biotechnologie de Strasbourg.
  • Since 2007 Associate Professor, School of Biological Sciences at NTU.

Major Research Interests

Biomolecular interactions, biosensors, drug discovery.

Research Interest

Surface Plasmon Resonance (SPR) based biosensors have become a forefront technology in monitoring biomolecular interactions in the last decade and is nowadays an indispensable tool in molecular biology research and drug discovery. It offers rapid, real-time detection without the need for labeling and allows precise quantification of binding parameters (kinetics, thermodynamics, affinity, stoichiometry) and concentration analysis, as well as qualitative applications (identification of binders, site specificity, epitope mapping, selectivity, drug screening,). We have established an SPR biosensor platform at SBS, in which we perform our own collaborative research and where the usage of the technique is available for interested users from SBS or other institutes in Singapore. Our projects involve characterization of different biomolecular interactions such as antibody/antigen to determine if a certain antibody can be used in immunotherapy, screening of interactions between target molecule and small molecules for drug discovery, characterization of different interactions in order to learn about cellular mechanisms, etc…

The main project in our lab is the study of the cytosolic AAA- ATPase p97/VCP that is involved in many cellular processes such as Endoplasmic Reticulum Associated Degradation (ERAD), Our aim has been to understand the interplay and regulation of p97/VCP ‘adaptor-preference’ regarding also disease-associated mutations in p97/VCP. SPR- biosensor technology has been very instrumental in measuring the different binding partners of p97/VCP and of the nucleotides to their respective binding sites. We are using the biosensor to address mechanistic questions by comparing binding responses of the different adaptor proteins under different conditions.

Signal generation Phases sensorgram Experimental example