Office: 01n-17 / 04s-31
Telephone: 6592 7506 / 6316 2856
- B.Sc. - Stockholm University (1976)
- Ph.D. - Stockholm University (1981)
- Post doctoral stay at University of Wisconsin-Madison, USA, 1982-1983.
- Faculty appointment at Stockholm University, Division of physical chemistry, 1983-2002.
- Full professor in physical chemistry at Stockholm University, from 2000.
- Professor at School of Biological sciences, NTU, Singapore from 2002.
Chromatin Biochemistry and Biophysics: Mechanisms and Regulation of Chromatin Compaction
A general aim of our research is the unravelling of the principles and control of the packaging of DNA in the cell nucleus within the context of chromatin. We are interested in understanding the electrostatic mechanisms and the molecular interactions that induce, stabilize and regulate chromatin folding and the implications of this for gene regulation. One central question is: How is chromatin folding and aggregation affected by posttranslational modifications that change the charge of the histone N-terminal tails.
Some specific aims are:
- To experimentally and theoretically investigate the folding of recombinant in vitro prepared nucleosome core particles (NCP) and well-defined chromatin in the form of nucleosome arrays and how this is affected by changes of the histone tail charges.
- To establish the physical basis in terms of electrostatic/molecular interactions for DNA interactions, structure and dynamics within the context of chromatin using modelling tools.
These aims are realized by using a combination of molecular biology, biochemical and chemical synthesis techniques in combination with biophysical approaches such as single molecule measurements (magnetic tweezers), solid-state NMR, synchrotron x-ray diffraction (SAXS), analytical ultracentrifugation, as well as well as Electron (EM) and other microscopy methods. Experimental work is often performed in combination with computer modelling studies.
and biophysical investigations of the effects of histone tail modifications on
folding and fibre self-association in chromatin.
- Telomeric chromatin: unravelling the structural properties of telomeric chromatin and how it differs from non-telomeric chromatin. We are part of the interdisciplinary international Telomere Dynamics Group (TDG) research programme (here).
- Multi-scale computer modeling of electrostatic interactions in DNA, the nucleosome core particle (NCP) and chromatin.
Illustration of the systems studied in biophysical, biochemical and computer modeling studies in this project. Upper panel: the nucleosome core particle. Lower panel: a 12-mer nucleosome array (chromatin).