Proteomics and Mass Spectrometry Services

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Proteomics and Mass Spectrometry Services

  • Maldi

    MALDI TOF/TOF
    ABI 4800

  • ESI

    ESI-MS
    Thermo Q Exative

  • ESI

    ESI-MS
    Agilent 6530 Q-TOF

  • Orbitrap

    Linear Ion trap Orbitrap
    Thermo LTQ-Orbitrap

  • FTICR

    Linear Ion trap FTICR
    Thermo LTQ-FT

The Proteomic Core Facility of the Biological Research Center is please to offer mass spectrometry services to the NTU community and beyond. We provide a wide range of services including molecular weight determination for biomolecules and protein identification from various sources, and are hoping to expand further in the near future.

To request a service, please fill in an appropriate sample submission form and email to SBSMS@ntu.edu.sg. Please also print out a hard copy to attach to each sample and submit to Meng Wei at Mass Spec room, Room No: SBS-03N-S1 at level 3N.

We are committed to provide high quality service in affordable cost, with fast turnaround under normal instrumental conditions. We guarantee protein (unlabeled) identification from coomassie blue-stained gel bands, or the service will not be charged.

Contacts

Email Phone
Meng Wei MengWei@ntu.edu.sg 65922639 (03N-S1)
Sze Siu Kwan, Newman sksze@ntu.edu.sg 65141006

Sample Submission Forms

Mass Spectrometry Service Charge

Services NTU Users Other academic users Non-academic users
Packaged service (including sample preparation, database searching)
Protein ID by MALDI-TOF/TOF $100/sample $160/sample $240/sample
Protein ID by LC-MS/MS $210/hour $300/hour $440/hour
4-plex iTRAQ with reagent and sample preparation $3000 $4500 $6000
6-plex TMT with reagent and sample preparation
$4100 $6150 $8200
10-plex TMT with reagent and sample preparation
$4800 $7200 $9600
Whole proteome ID (including sample preparation, HPLC fractionation) $2500 $3750 $5000
Phosphopeptide enrichment and analysis $300/protein (sample preparation $150); $2500/phosphor-proteome (sample preparation $500) $400/protein; $3750/phosphor-proteome $600/protein; $5000/phosphor-proteome
N-Glycopeptide enrichment and analysis $300/protein; $2500/glycol-proteome $400/protein; $3750/phosphor-proteome $600/protein; $5000/phosphor-proteome
SILAC (inlcuding HPLC fractionation $350/sample; $2500/proteome $500/sample; $3500/proteome $700/sample; $5000/proteome
Ready for run samples (option for those performing own sample preparation)
Protein ID by MALDI-TOF/TOF $30/sample $60/sample $100/sample
Protein ID by LC-MS/MS $150/hour $200/hour $300/hour
Molecular Weight determination by MALDI-TOF/TOF $20/sample $30/sample $40/sample
Molecular Weight determination by LTQ-Orbitrap/LTQ-FT/Q-TOF $150/sample $200/sample $300/sample
Other individual service
Desalting/Sample concentration $20/sample $25/sample $40/sample
LCQ Service Charge (SBS users only)
M.W. by injection/infusion – performed by user $40/hour - -
LC/MS/MS analysis - performed by user $40/hour - -
MALDI Service Charge (SBS users only)
MALDI-TOF/TOF performed by user  $160/hour - -
MRM Service Charge 
Sample Preparation $60/sample $90/sample
$120/sample
MRM (first sample)  $100/protein $150/protein
$200/protein​
Subsequent sample with same transition list 50% off 50% off
50% off
Q-TOF Service Charge 
Metabolite/other experiment performed by user (SBS users only) $100/hour - -
Metabolite/other expts performed by staff $150/hour $200/hour
$300/hour

Note:

  1. Service charge may be changed at the discretion of School of Biological Sciences without prior notice.
  2. All the service charge is before GST.
  3. For proteome/iTRAQ/TMT samples, the price is for running once, if additional repeat is required, additional charge will apply. Repeat will give 50% discount 
  4. LCMS sample, if converted from MALDI-TOF, if sample quantity is more than 10, 20% discount will be given. If sample quantity is more than 20, 30% discount will be given.
  5. Normal LCMS sample, if sample quantity is more than 10, 10% discount will be given. If sample quantity is more than 20, 20% discount will be given.
updated 2016.08.01​


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