Cell Biology Core Facility

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FACS

Flow Cytometry has the ability to discriminate, quantify and sort distinct population of cells based on expression of markers with rapid ease thus opening up new research opportunities or aid in current research work. A summary of how flow cytometry is used is found here.
BD FACSCalibur™
DSC00260.JPG Specifications
  • Location: 02n-23 (Teaching Lab 3).
  • Up to 4-colour detection
  • Digital acquisition and analysis system
    2 air-cooled lasers at 488-nm Blue, 633-nm Red
Application
  • For detection and analysis of cells or other microorganisms.
  • Eg. Cell cycle analysis, Apoptotic studies, DNA analysis
BD LSR II

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Specifications
  • Location: 02s-69 (Flow Cytometry Room)
  • Up to 9-color detection
  • Digital acquisition and analysis system
  • 4 air-cooled lasers at 405-nm Violet, 488-nm Blue, 561-nm Yellow/Green and 633-nm Red.

 

Application
  • For analysis of cells or other microorganisms
  • Eg. DAPI staining, Calcium Flux Analysis.

                                                                                     

 

                                                                           BD FACS Aria 1 Sorter

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Specifications
  • Location: 02s-69 (Flow Cytometry Room)
  • multicolor analysis for up to 7 parameters
  • 2 air-cooled lasers at 488-nm Blue, 633-nm Red
  • Digital acquisition rates of up to 70,000 events/second
  • Two- and four-way bulk sorting devices for a variety of tube sizes
Additional Option

Automated Cell Deposition Unit (ACDU) for sorting to multi-well platesand microscopic slides

 

Application
  • For non-sterile sorting of bacteria, yeast  and algae.
  • Direct sort (single-cells or bulk) into PCR tubes for RNA preparation work
  • High-speed cell sorting and single cell sorting into 96 well plate. 

BD FACS Aria 3 Sorter
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Specifications
  • Location: 02s-68 (Flow Cytometry Room)
  • multicolor analysis for up to 15 parameters
  • 4 air-cooled lasers at 355-nm UV, 488-nm Blue, 561-nm Yellow/Green and 633-nm Red.
  • Digital acquisition rates of up to 70,000 events/second
  • Two- and four-way bulk sorting devices for a variety of tube sizes
Additional Option
Automated Cell Deposition Unit (ACDU) for sorting to 96 multi-well plates and microscopic slides.
 
Application
  • Only for for sterile sorting of mammalian cells and liposomes.
  • Direct sort (single-cells or bulk) into PCR tubes for RNA preparation work
  • High-speed cell sorting and single cell sorting into 96 well plate. 

 

BD 3-Lasers Fortessa X20 with High-Throughput Sampler (HTS)

Specifications
  • Location: 02s-69 (Flow Cytometry Room)
  • multicolor analysis for up to 10 parameters
  • 3 air-cooled lasers at 355-nm UV, 488-nm Blue and 633-nm Red.
  • Digital acquisition rates of up to 70,000 events/second
  • HTS option.

3 LASERS FORTESSA X20 with HTS.jpg3 LASER CONFIG.jpg



 

 
 

 

                                                                     BD 5-Lasers Fortessa X20
 
 
Specifications
  • Location: 02s-69 (Flow Cytometry Room)
  • multicolor analysis for up to 14 parameters
  • 5 air-cooled lasers at 355-nm UV, 405-nm Violet, 488-nm Blue, 561-nm Yellow/Green and 633-nm Red.
  • Digital acquisition rates of up to 70,000 events/second

 

5 LASERS FORTESSA X20.jpg
5 LASERS CONFIG.jpg
 

Charging

FACS charges.jpg

Procedures for use of equipment

  • Internal Users are to use Online Booking System and book their usage at least a day in advance
  • New users from Nanyang Technological University are to email the contact in-charge below.
  • Users are encouraged to email the contact in-charge below for discussion of experiments and use of equipment

For enquiries, please contact

Scientific consultant and in-charge: Christiane, Ruedl (Associate Professor) (mailto:Ruedl@ntu.edu.sg)

 

 

Assistant Manager / FACS Consultant and training: Abdul Rashid B M Muzaki (Dr) (arashid@ntu.edu.sg)

                                                 

 

 


 

 

 

 

 

 Documents

 
  
  
  
  
3 LASER CONFIG.jpg
  
4/18/2018 11:24 AMNo presence informationAbdul Rashid Bin Mohammad Muzaki (Dr)
3 LASERS FORTESSA X20 with HTS.jpg
  
4/18/2018 11:11 AMNo presence informationAbdul Rashid Bin Mohammad Muzaki (Dr)
5 LASERS CONFIG.jpg
  
4/18/2018 11:32 AMNo presence informationAbdul Rashid Bin Mohammad Muzaki (Dr)
5 LASERS FORTESSA X20.jpg
  
4/18/2018 11:29 AMNo presence informationAbdul Rashid Bin Mohammad Muzaki (Dr)
FACS charges.jpg
  
4/18/2018 11:50 AMNo presence informationAbdul Rashid Bin Mohammad Muzaki (Dr)
Fortessa 3 Laser CONFIG.pdf
  
4/18/2018 11:17 AMNo presence informationAbdul Rashid Bin Mohammad Muzaki (Dr)
Uses of flow cytometry-edited.pdf
  
5/31/2013 11:22 AMNo presence informationTeng Wei Heng